Stable concentrated preparation for the indigo fermentation-vats.



barman r clarion ALBRECHT SCHMIDT, F HijCHSTON-TI-IEMAII T, GERMANY, ASSIGNOR T0 FAEIBWERKE VORM. MEISTER LUCIUS BR'UNING, OF H6CHST'0N-THEMAIN, GERMANY, A COR- ?GRATION 0F GERMANY.

S'EMLE GONCENTRA'IEED "PREPARATION FOR THE INDIGO -IiERMENTATION-VATS.

'll,239,52fi.

Ito Drawing.

' useful Improvements in Stable Concentrated Preparations for the Indigo Fermentation- Vats, of which the following 1s a specification.

v lndigo-vats enous bodies,

The action of the socalled cold oriental is due, for-the most part, to phenomena oi fermentation. They are caused by bacteria whichrequire for their existence and growth nitrogenous nutrient media. and consequently it has been found that by adding fermentable but nonnitrogsuch as glucose, cane sugar, starch, etc., a cold fermentation-vat cannot be satisfactorily started and continuously kept working. In practice there has already for a long time been used :as fermentable materials as they were called,besides syrup, starch, glucose, etc., also bran, which, as is known, contains vegetable albumin e. nitrogenous material. It has been attempted to mix stable-indigo and indigo-white pastes with vat-fermentation agents with the addition of alkalis in order to convert them thereby into a stable form. Thus, according to a well known process 200 parts of indigo are made into a paste with 100 parts of wheat-flour, parts of syrup, 10 parts of quick lime and 540 parts of water. This process shows, however, the disadvantage that the paste thus obtained becomes putrefied and ferments as soon as the alkaline reagent disappears for some-reason, for in stance in consequence of the lime being consumed by the carbonic acid in the air.

Now I have found that stable concentrated albumin preparations can be obtained which retain their action in the cold indigo fermentation-vat, by mixing isolated albumin substances, containing or not containing bacteria, with water-soluble carbohydrates, for instance with molasses, glucose or the like, orwith equivalents of water-soluble carbohydrates of high concentration lor instance glycerin. Thus valuable stable albuminpreparations are obtained either by .(IllXlIlg' a concentrated water-soluble carbohydrate, for instance With the moist extract contain- Specification of Letters Patent.

Application filed. November 13, 1914. Serial No.

Patented sepia. til, 191 "Z.

ing a high percentage of albumin (gluten), obtainable by extracting or boiling bran or an albuminous seed with water and, if required, subsequently evaporating; or, for instance, with other albuminous substances (albuminates), such as vegetable casein, casein or the like and also with albuhwses, peptonoid products or the like. The high percentage of sugar causes preservation of the albuminous material in spite ofits containing water and the sugar itself serves then as one of the fermentation agents in the vat. By such preparations it is possible to start well the so-called cold lime-fermentation-vat and to keep it working. The indigo-vats prepared with such albuminous and sacchariferous products show a considerably better condition than those prepared for instance with glucose without said albuminous products added; the deposit of the vat, when agitated, shows a pure yellow color which is not the case with the ordinary glucose-limo vat; the vat has not so great a tendency to deposit, etc, and works in many respects better than the ordinary glucose- ]ime-vat owing to the albumin it contains.

These mixtures of albuminous extracts and concentrated water-soluble carbohydrates have the following properties: They give the chemical reactions of the albumins (biuret reaction) and also those of the watersoluble carbohydrates (in the inverted state, reduction of Fehlings solution) when exposed to the air or left at rest, they do not putrefy but when diluted with water putre-' faction sets in at once; when introduced into the indigowat, they revive the latter, 6. they set it inaction that is to say, the indigo is soon reduced. It with such liquid albumi' nous carbohydrates are incorporated at the same time indigo or indigo white or the known indigo-white mixtures, for instance mixtures of indigo-white and zinc dust and carbohydrates, there are obtained also indigoand leuco-indigo preparations which can be directly used.

By water-soluble carbohydrates are to be understood in the foregoing specification the water-soluble carbohydrates as such and, of course also glycerin, this being an entire technical equivalent thereof.

Example: 10 kg. of rye-bran are exsuitable for transport; it does not and dry up. The respective incorporate indigo with all trated pastes S0 drates, such as tained Concentrated, stable 9 haps be contained in of a fermentation liquor I butyric acid; by using the concentrated Sugar solution these bacteria 5 luted, is then evaporated, preferably in a 11, vacuum, so as to turn into a syrup-like mass which is then mixed with 3 kg, or more or less, of molasse; according as required. The paste thus obtained is indefinitely stable and putreiiy albuminous substance may be incorporated with the watersoluble carbohydrate also in'a more purified or purer form by using for instance instead 15 of the extract or the gluten, the vegetable casein produced therefrom or another albuminate or other casein-like substances, albumoses, peptonoid products or the like or also their salts. If it is intended to of these albuniinous carbohydrate-pastes,

it is only adcase the the same as above indicated; vantageous to increase in this maltose, glycerin etc.

indigo-white is added to the concenproduced from liquid albuminous extracts and water-soluble carbohylnolasses, invertsugar, gluglyoerin, etc, there are obindigo white cose, maltose,

products.

When preparing solid prepai'ationswitn :35. or without adding indigo or indigo-white the mixture is evaporated. to dryness or ukilatized, preferably in occur) or in a current of air, so that the last remainder of water is removed. The water-soluble carloohydrate contained therein renders it also possible, owing to the sugar it contains, to easily transform substance into a paste when the respective preparations are moistened with water, to

. 5 retain the substance in a hydrated state and to prevent it from sticking together.

In making such concentrated albuminous carbohydrate preparations there may be added, besides the bacteria which may perthe albuminous raw products themselves, other materials containing bacteria, such for instance as finely cut hay, or hay-tea or the like or portions of lactic acid or are at the same time rendered latent and preserved.

4 p the proce' dureis apart from the addition of indigo the nitrogenous organic incense Having now described my invention, what 1' claim is:

1. In the art of indigo dyeing, the process of reviving the indigo vat and reducin the indigo, which consists in. adding to the bath a mixture containing'a concentrated albuminous product and a;.water-solubl concentrated carbohydrate, said mixture characterized by giving the chemical reactions of the two components, viz., the albuminand sugar-reactions, and-having the property of not putrefying when exposed to the air but of undergoing putrefa'ction when diluted with water.

As new products, mixtures of concentrated albuminous extracts and Watersoluble carbohydrates and indigo,which mixtures are characterized by giving the chemical reactions of {the components, namely the albumin-, sugarand indigo-reactions, having the property of dyeing m a reduced state cotton and wool a blue color, of not putrcifying when exposed to the air but un- (lei-going putrefaction when diluted with water, and of dyeing, when diluted with wate' .and rendered alkaline by means of lime, after some time, cotton and wool blue tints.

3. As new products, mixtures of conccntrated albuni'iuous extracts and Watersoluble carbohydrates and indigo-white, which mixtures are characterized by giving the chemical reactions of the components, namely the albumin, sugarand indigowhitc rea ctions; having the property of dyeing in an alkaline solution cotton and Wool a bluecolorylof not putrefying when exposed to the air but undergoing putrefaction when dilutedv'with water, and of dyeing, when diluted with water and rendered alkaline by means of lime, cotton and wool. blue tints. I

4, As a new product, a solid, substantially water-free acomposition consisting essentially oi" indigo, a water-soluble carbohydrate, and" an albuminous substance.

As at new product, a solid-substantially am-rm composition consistin essentially otYindigo-white, a water-soluble carbohydrate, and an albuminous substance.

ll n testin'iony whereof I aiiix my signature in presence of two witnesses.

ALBRECHT SQHMIDT.

. Witnemes:

i5 1 JEAN GRUND, CARL GRUND. 

